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Leadgene Leadgene
Anti-IL-4 Antibody [Clone 6-5]
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Catalog Number

LDG0023YA

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  • Overview

    Description

    Interleukin-4 (IL-4) is a multifunctional anti-inflammatory cytokine produced by T helper 2 (TH2) cells, plays an important role in the allergic inflammation. This protein induces differentiation of naive helper T cells (TH0 cells) to TH2 cells, IgG class switch to IgE in B cells and alternative macrophage activation.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:400-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 6-5

    • Immunogen

      Interleukin 4

      Reactivity

      Human

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      Interleukin 4

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-IL-4 Antibody [Clone 6-5] MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-IL-4 monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-IL-4 Antibody [Clone 6-5]
    MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
    Triton X-100. Cells were stained with mouse anti-IL-4 monoclonal antibody
    (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
    1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    FACS analysis of Anti-IL-4 Antibody [Clone 6-5] MCF-7 cells were stained with mouse anti-IL-4 monoclonal antibody at 1 μg/ml (red) and without antibody control (black).

    FACS analysis of Anti-IL-4 Antibody [Clone 6-5]
    MCF-7 cells were stained with mouse anti-IL-4 monoclonal antibody at 1
    μg/ml (red) and without antibody control (black).

    ELISA titration of Anti-IL-4 Antibody [Clone 6-5] Titration curve of anti-IL-4 antibody in ELISA. Red: IL-4; Black: BSA (negative control).

    ELISA titration of Anti-IL-4 Antibody [Clone 6-5]
    Titration curve of anti-IL-4 antibody in ELISA. Red: IL-4; Black: BSA
    (negative control).

    Western blotting analysis of Anti-IL-4 Antibody [Clone 6-5]

    Western blotting analysis of Anti-IL-4 Antibody [Clone 6-5]

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  • Datasheet & Documents
    MSDS
    Manual
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