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Catalog Number

LDG0023YA

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Overview

Description

Interleukin-4 (IL-4) is a multifunctional anti-inflammatory cytokine produced by T helper 2 (TH2) cells, plays an important role in the allergic inflammation. This protein induces differentiation of naive helper T cells (TH0 cells) to TH2 cells, IgG class switch to IgE in B cells and alternative macrophage activation.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:400-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG1
  
  Clone Name
  
  clone 6-5
- Immunogen
  
  Interleukin 4
  
  Reactivity
  
  Human
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  Interleukin 4
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-IL-4 Antibody [Clone 6-5]
MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
Triton X-100. Cells were stained with mouse anti-IL-4 monoclonal antibody
(1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

FACS analysis of Anti-IL-4 Antibody [Clone 6-5]
MCF-7 cells were stained with mouse anti-IL-4 monoclonal antibody at 1
μg/ml (red) and without antibody control (black).

ELISA titration of Anti-IL-4 Antibody [Clone 6-5]
Titration curve of anti-IL-4 antibody in ELISA. Red: IL-4; Black: BSA
(negative control).

Western blotting analysis of Anti-IL-4 Antibody [Clone 6-5]

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Datasheet & Documents

Datasheet

MSDS

Manual

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