Anti-SARS-CoV-2 Spike Antibody [Clone LGSV201]

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Catalog Number

LDG0086YA

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Overview

Description

Human anti-SARS-CoV-2 Spike Antibody [CR3022] recognizes human SARS-CoV and CoV-2 Spike protein with high affinity. The binding site is amino acids 318-510 (RBD, Receptor Binding Domain) in the S1 subunit of the spike protein. Coronavirus spike protein conducts the process that interacts with cellular receptor and membrane fusion to allow virus entering into target cells. Spike protein also can be used to define the specificity of the virus and be used as a critical target for vaccine design. The glycosylated spike protein can be detected in the virus-infected cell and cell culture medium. The RBD is responsible for recognizing the cell surface receptor.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB:1: 1000-5000
IFA:1: 500-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG1
  
  Clone Name
  
  clone LGSV201
- Immunogen
  
  SARS-CoV-2 VLP
  
  Reactivity
  
  SARS-CoV & CoV-2
- Application
  
  ELISA, WB, IFA
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  Spike protein
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Western blotting analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:5000)
Lane: SARS-CoV-2 VLP

LDG0086YAWB01 Western blotting analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:5000)Lane 1: SARS-CoV-2 S1-RBDLane 2: SARS-CoV-2 S1Lane 3: SARS-CoV S1-RBDLane 4: SARS-CoV S1Lane 5: MERS-CoV S1-RBD

Immunofluorescence analysis of Mouse anti-SARS-CoV-2 Spike mAb (1:1000)

ELISA titration of Mouse anti-SARS-CoV-2 Spike mAb

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Datasheet & Documents

Datasheet

MSDS

Manual

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