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Catalog Number

LDG0047YA

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Overview

Description

Transforming growth factor beta (TGF-β), a polypeptide member of the transforming growth factor beta superfamily of cytokines that includes three isoforms, is involved in various biological processes. TGF-β1, the most abundant TGF-β isoform, is a secreted protein that plays important role in cell proliferation, differentiation and apoptosis and is involved in wound healing, inflammatory processes and prevention of tumor growth.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-10000
IFA: 1:400-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG1
  
  Clone Name
  
  clone 51-3
- Immunogen
  
  TGF Beta 1
  
  Reactivity
  
  Human
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  TGF Beta 1
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
293T cells were respectively transfected with TGF beta 1, beta 2 and beta 3
plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1%
Triton X-100. Cells were stained with mouse anti-TGF beta 1 monoclonal
antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-
iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342
(Blue).

FACS analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
293T cells (black) were respectively transfected with TGF beta 1 (red), beta
2 (blue) and beta 3 (green) plasmid and stained with mouse anti-TGF beta 1
monoclonal antibody at 1 μg/ml.

ELISA titration of Anti-TGF Beta 1 Antibody [Clone 51-3]
Titration curve of anti-TGF beta 1 antibody in ELISA. Red: TGF beta 1;
Black: BSA (negative control).

Western blotting analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
Recombinant proteins and TGF beta 1, 2 or 3 transfected 293T cell lysates
were stained with mouse anti-TGF beta 1 monoclonal antibody at 1:2000
dilution. Lane 1: recombinant TGF beta 1 protein (25 ng). Lane 2:
recombinant TGF beta 2 protein (25 ng). Lane 3: recombinant TGF beta 3
protein (25 ng). Lane 4: TGF beta 1 transfected 293T cell lysates (30 μg).
Lane 5: TGF beta 2 transfected 293T cell lysates (30 μg). Lane 6: TGF beta
3 transfected 293T cell lysates (30 μg).

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Datasheet & Documents

Datasheet

MSDS

Manual

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