Anti-V5 Tag Antibody [Clone 7-1-8]

Catalog Number

LDG0003YB

Datasheet

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• Overview

  Description

  Mouse monoclonal anti-V5 antibody is an antibody specific to GKPIPNPLLGLDST (V5) tags which can be applied to several immunoassays including ELISA, WB, IHC, and Flow cytometry.
  This antibody has been identified in several sources (bacteria, yeast, and mammalian cells) of V5-fusion protein.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IP: 1:500-2000
  IFA: 1:500-1000
  FACS: 1:500-1000
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2b

    Clone Name

    clone 7-1-8

  • Immunogen

    Pepetide: GKPIPNPLLGLDST (V5)

    Application

    ELISA, WB, IP, IFA, FACS

  • Conjugation

    Unconjugated

    Concentration

    1 mg/mL

  • Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    Specificity

    Recognizes tag sequence of GKPIPNPLLGLDST on either the N- or C-terminus of recombinant fusion protein.

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Western blotting analysis of anti-V5 tag mAb (1:5000)
  Lane 1: 293T whole cell lysate-untransfected
  Lane 2: 293T whole cell lysate-transfected
  Lysate/proteins at 20 μg per lane

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.