D-3-Hydroxybutyrate dehydrogenase (BDHA)

Catalog Number

LDG0022RG

Datasheet

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• Overview

  Description

  D-3-Hydroxybutyrate dehydrogenase (BDHA) is an enzyme crucial in the metabolism of ketone bodies, specifically involved in the conversion of D-3-hydroxybutyrate to acetoacetate during ketogenesis. BDHA plays a vital role in energy production, particularly under conditions such as fasting or prolonged exercise when ketone bodies serve as alternative energy sources. This enzyme is essential for maintaining metabolic balance and is found in various tissues, including the liver and kidneys. Understanding BDHA's function is significant for insights into metabolic disorders and energy metabolism regulation.

• Specifications

  • Expression system

    Escherichia coli

    Application

    1. Enzymatic determination of ketone bodies (D-3- Hydroxybutyrate)
    2. Detection of the presence of ketone bodies

  • Detection Method

    Spectrophotometry

    Concentration

    300 U/ mg (containing approx. 10% of stabilizers)

  • Buffer

    Lyophilized from a 0.2 µm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.5.

    Purity

    >95% as determined by SDS-PAGE analysis.

  • Activity

    Please refer to the manual for details.

    Unit Definition

    One unit causes the formation of one micromole of NADH per minute under the conditions described in the manual.

  • Reaction Condition

    0.1 M Tris-HCl pH 8.5, 25 mM 3-Hydroxybutyrate, 1.8 mM NAD⁺

    Form

    Lyophilized

• Instruction

  • Reconstitution

    It is recommended to weight 10 mg of lyophilized powder, reconstitute in 250 μL double-distilled water directly (final activity is 40 U/ μL), and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C or -80°C for long-term storage under sterile conditions.

    Avoid repeated free-thaw cycles.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.