EGFP, His Tag, E. coli

Catalog Number

LDG032PVE

Datasheet

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• Specifications

  • Species of Origin

    Human cytomegalovirus (HHV-5) (Human herpesvirus 5)

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 27.99 kDa.
    The protein migrates as 27 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin level

    <1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    Enhanced green fluorescent protein (EGFP), also known simply as GFP, is a protein renowned for its ability to emit a brilliant green fluorescence upon exposure to light within the blue to ultraviolet spectrum. Notably, EGFP possesses a remarkable feature: it can autonomously form an internal chromophore without necessitating any additional enzymes, substrates, cofactors, or gene products aside from molecular oxygen. This characteristic makes EGFP an ideal candidate as a reporter gene. Researchers frequently employ EGFP to express it throughout an organism, in specific organs or selected cells of interest, often utilizing transgenic techniques. Importantly, the introduction of EGFP into an organism's genome or that of its offspring does not induce any genomic alterations. EGFP has been successfully expressed across a diverse array of species, spanning from bacteria, fungi, and yeast to mammals, including human cells.

    Uniprot ID

    GenBank: AAK15492.1

  • Sequence Note

    Met1-Lys239

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of EGFP protein.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.