Description
Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.
Leadgene® Endoglycosidase H (Endo H) ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Endo H level in sample solution. The Endoglycosidase H ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.
Test Principle
Endoglycosidase H ELISA kit is used to detect Endo H in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-Endo H monoclonal antibody to the solid phase. Endo H in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-Endo H monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of nuclease level is accomplished by comparing the absorbance with standard curve.
