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Species of Origin
Streptococcus pyogenes
Expression system
Escherichia coli
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Affinity Tag
His-MBP Tag (N-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 201 kDa.
The protein migrates about 180 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
The enhanced specificity Cas9 protein from Streptococcus pyogenes (~160 KD), modified at sites K848A/K1003A/R1060A, exhibit substantially reduced off-target cleavage in cells. When combined with target RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and help the development of genetically-modified animals via one-cell embryo injection.
Synonyms
CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9
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Uniprot ID
#BDU59491.1
Sequence Note
Asp41-Asp1407
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![SDS-PAGE analysis of recombinant eSpCas9 (1.1).]()
SDS-PAGE analysis of recombinant eSpCas9 (1.1).
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Review
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Publication
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Datasheet & Documents
1/1
Disclaimer：For Research Use or Further Manufacturing Only.
