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Species of Origin
Human coronavirus 229E
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS containing 500 mM NaCl, pH 7.4.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 44.28 kDa.
The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis). -
Form
Lyophilized
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Specifications
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Background
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Background
There are seven human coronaviruses have been identified. The common human coronaviruses are four groups, known as 229E (α coronavirus), NL63 (α coronavirus), OC43 (β coronavirus) and HKU1 (β coronavirus). Because the crown-like spikes on the surface of virus, they are named for coronaviruses. HCoVs cause the respiratory tract diseases, especially severe in infants and the elderly. The spike protein controled the infection of target cells and it facilitated entry into cells by binding cellular receptors.
Synonyms
Nucleoprotein, Nucleocapsid protein, NC Protein N
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Uniprot ID
#P15130
Sequence Note
Met1-Asn389
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Review
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.