Description
Leadgene® Hot Start Taq DNA Polymerase contains Taq DNA Polymerase and an anti-taq monoclonal antibody which blocks polymerase activity. Enzyme activity is recovered during the initial incubation step while the taq antibody is denatured and dissociates from the DNA polymerase. Hot Start Taq DNA Polymerase exhibits higher specificity, sensitivity, and yield by reducing non-specific amplification and primer-dimers. This enzyme possesses 5’→3’ polymerase activity and 5’ → 3’ exonuclease replacement activity, but lacks a 3’ → 5’ exonuclease (proof-reading) activity. Hot Start Taq DNA Polymerase is suitable for most PCR applications. The enzyme formulation does not contain glycerol and is compatible for further lyophilization process.
Components
500 U
Items
Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)
Quantity
1 vial (500 U)
Items
10× Hot Start Taq Buffer (Mg²⁺ free)
Quantity
1 vial (1 mL)
Items
25 mM MgCl₂
Quantity
1 vial (1 mL)
1,000 U
Items
Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)
Quantity
1 vial (1,000 U)
Items
10× Hot Start Taq Buffer (Mg²⁺ free)
Quantity
2 vials (1 mL)
Items
25 mM MgCl₂
Quantity
2 vials (1 mL)
