HRV 3C Protease

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Catalog Number

LDG0009RG

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• Overview

  Description

  HRV 3C Protease is a recombinant form of the 3C protease derived from human rhinovirus 14 expressed in E. coli (specific activity 1800-2000 U/mg). This product is a highly purified recombinant 6XHis-fusion protein. This protease requires neither metal nor cofactors for activity. HRV 3C Protease recognizes the cleavage site: Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP).

  Product Note

  • Cleavage efficiency may differ based on structure and properties of each target protein, we recommend testing several enzyme-to-substrate ratios, temperatures and incubation times.
  • HRV 3C Protease reactions can be performed in a buffer which is optimal for the target protein. Reducing reagents (e.g., DTT) or salts (e.g., NaCl) can be added for cleavage efficiency evaluation.

  Components

  Package	Items	Quantity
  1,000 U	HRV 3C Protease	1 vial (1,000 U)
  	10X HRV 3C Cleavage Buffer	1 vial (10 mL)
  10,000 U	HRV 3C Protease	1 vial (10,000 U)
  	10X HRV 3C Cleavage Buffer	1 vial (10 mL)

  • 1,000 U

    Items

    HRV 3C Protease

    Quantity

    1 vial (1,000 U)

    Items

    10X HRV 3C Cleavage Buffer

    Quantity

    1 vial (10 mL)

  • 10,000 U

    Items

    HRV 3C Protease

    Quantity

    1 vial (10,000 U)

    Items

    10X HRV 3C Cleavage Buffer

    Quantity

    1 vial (10 mL)

• Specifications

  • Expression system

    Escherichia coli

    Buffer

    Lyophilized from a solution containing 50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.04% Tween20, 8% trehalose, 8% mannitol.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Unit Definition

    One unit of HRV 3C Protease is defined as the amount of enzyme that will cleave >95% of 0.1 mg HRV 3C cleavage control protein in 150 mM NaCl, 50 mM Tris-HCl pH 7.5, at 4°C for 16 h.

  • Endotoxin level

    <1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  HRV 3C protease removes the purification tag appended to interest proteins after the affinity chromatography step.

  SDS-PAGE analysis of recombinant HRV 3C Protease Protein

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• Review

  

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• Publication

  1. Yang MC et al. PLoS One. 2023; 18(3):e0283473.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.