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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 14.43 kDa.
The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.7 ng/mL. The specific activity of recombinant human beta-NGF is > 1 x 10⁶ IU/mg.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Nerve Growth Factors (NGF) is critical for the development and maintenance of the sympathetic and sensory neuron systems. NGF has been demonstrated as a complex that consists of three polypeptides named α, β and γ subunits. Among then, β subunit, which known as beta-NGF is a 26.9 kDa protein containing 241 residues that involve in neuronal survival and differentiation. Besides, beta-NGF also acts as a ligand to TRKA receptor, which indispensable for the differentiation and development of pain and temperature sensing neurons.
Synonyms
Nerve Growth Factors-Beta, Beta-NGF
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Uniprot ID
#P01138
Sequence Note
Ser122-Ala241
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![Human Beta-NGF, His Tag, E. coli (LDG129PHE) induced TF-1 cell proliferation, with the ED50 at 0.6093 ng/mL.]()
Human Beta-NGF, His Tag, E. coli (LDG129PHE) induced TF-1 cell proliferation, with the ED50 at 0.6093 ng/mL.
![SDS-PAGE analysis of recombinant human beta-NGF.]()
SDS-PAGE analysis of recombinant human beta-NGF.
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Review
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
