Human BMP-12, His Tag, E. coli

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Catalog Number

LDG110PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 14.95 kDa.
    The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is < 2 μg/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Bone Morphogenetic Protein-12 (BMP-12), known as Growth differentiation factor 7 (GDF7), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-12 can bind with the TGFβ receptor and trigger SMAD protein signal transduction. BMP-12 is in a signaling center called the roof plate in the embryo's developing nervous system, where it sends signals to induce the generation of sensory neurons.

    Synonyms

    Growth/differentiation factor 7

  • Uniprot ID

    #Q7Z4P5

    Sequence Note

    Lys352-Arg450

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  

  Human BMP-12, His Tag, E. coli (LDG110PHE) induced alkaline phosphatase production by ATDC5 cells, with the ED50 at 111.8 ng/mL.

  

  SDS-PAGE analysis of recombinant human BMP-12.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.