Human CD326, His Tag, E. coli

Catalog Number

LDG066PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 28.36 kDa.
    The protein migrates as 30 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by the ability of the immobilized protein to support the adhesion of the 3T3 cells. The ED₅₀ for this effect is 0.2-1.7 ng/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Human CD326 also called epithelial cellular adhesion molecule (EpCAM), which is a 28 kDa protein containing 242 amino acid residues. Human CD326 is a transmembrane glycoprotein, which is a marker of cancer. CD326 induces the expression of c-myc, cyclins A & E and e-fabp which is involved the cancer cell proliferation and migration

    Synonyms

    Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial glycoprotein , EGP, Epithelial glycoprotein 314 , EGP314,  hEGP314, KS 1 /4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Epithelial cell adhesion molecule, Ep-CAM

  • Uniprot ID

    #P16422

    Sequence Note

    Gln24-Lys265

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human CD326.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.