Human CD40L, His Tag, E. coli

Catalog Number

LDG116PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 16.99 kDa.
    The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <5 ng/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Human CD40L (CD154) is one member of TNF family which is expressed on T cells. It binds the CD40, which is on the antigen-presenting cells (ex: B cells, macrophages and monocytes) Human CD40L is a 21.6 kDa cytokine with 103 amino acid residues which is a transmembrane glycoprotein. Human CD40L plays an important role in the adaptive immune system which induces the B cell maturation and activation.

    Synonyms

    CD40 ligand, CD40L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5

  • Uniprot ID

    #P29965

    Sequence Note

    Met1-Leu261

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human CD40L.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.