Human CXCL4, His Tag, E. coli

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Catalog Number

LDG156PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (N-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 8.58 kDa.
    The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to chemoattract human THP1 cells. Chemotactic activity was observed at a concentration of 10 ng/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    C-X-C motif chemokine 4 (CXCL4) also named platelet factor 4 (PF4), which is a chemokine of the intercrine alpha family. CXCL4 is a 8kDa protein containing 70 amino acid residues. CXCL4 is produced by the activated platelets which plays an important role in immune responses. CXCL4 inhibit the cell proliferation, platelet aggregation and wound repair. CXCL4 also suppresses the hematopoiesis.

    Synonyms

    C-X-C motif chemokine 4, Iroplact, Oncostatin-A, Platelet factor 4

  • Uniprot ID

    # P02776 2

    Sequence Note

    Glu32-Ser101

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human CXCL4, His Tag, E. coli (LDG156PHE) inhibited human FGF-2-induced HUVEC cell proliferation, with the ED50 at 4.87 μg/mL.

  SDS-PAGE analysis of recombinant human CXCL4.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.