Description
Fibroblast Growth Factors-1 (FGF-1) is a Growth Factors of mitogenic peptides which is a 15 kDa protein containing 139 amino acid residues. Fibroblast Growth Factors-1 is secreted by the macrophage which induce endothelial cell proliferation and angiogenesis. During wound injury, fibroblast Growth Factors-1 helps the tissue remodeling. When the fibroblast Growth Factors-1 binds with the FGFR on the cell membrane which is endocytosed by FGFR and induces the cell cycle.
Test Principle
Human FGF-1 ELISA kit is used to detect FGF-1 in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-human FGF-1 monoclonal antibody to the solid phase. Human FGF-1 in the sample conjugates on solid phase and then react with the HRP conjugated mouse anti-human FGF-1 monoclonal antibody. Subsequent wash steps will remove residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of nuclease level is accomplished by comparing the absorbance with standard curve.
