Human FGF-12, His Tag, E. coli

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Catalog Number

LDG077PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 21.23 kDa.
    The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 ng/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Fibroblast Growth Factors-12 (FGF-12) is a 20.4 kDa member of the fibroblast Growth Factors with 181 amino acid residues. FGF-12 is mainly expressed from inhibitory neurons, oligodendrocyte precursor cells, excitatory neurons, cardiomyocytes. Involved in nervous system development, function, and positive regulation of voltage-gated sodium channel activity. FGF-12 function in the development of the central and peripheral nervous systems, connective tissue of the skeleton, and the myocardia of the heart.

    Synonyms

    Fibroblast Growth Factors homologous factor 1, FHF-1, Fibroblast Growth Factors 12

  • Sequence Note

    #NP_004104.3 Met1-Thr181

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human FGF-12, His Tag, E. coli (LDG077PHE) induced 3T3 cell proliferation, with the ED50 at 1.61 ng/mL.

  SDS-PAGE analysis of recombinant human FGF-12.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.