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Leadgene Leadgene
Human GIF, His Tag, E. coli
Catalog Number

LDG061PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 46.23 kDa.
      The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Human Gastric intrinsic factor (GIF) is an intrinsic factor of the eukaryotic cobalamin family. Gastric intrinsic factor is a 44 kDa protein containing 380 amino acid residues. It is secreted from the parietal cells in gastric mucosa. Gastric intrinsic factor plays a major role in absorption and transportation of cobalamin and Cbl. It could form the comprised with Vit.B12 that avoid the destroy of Vit.B12.

      Synonyms

      Cobalamin binding intrinsic factor, Gastric intrinsic factor, Intrinsic factor

    • Uniprot ID

      #P27352

      Sequence Note

      Met1-Tyr417

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human GIF.

    SDS-PAGE analysis of recombinant human GIF.

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  • Datasheet & Documents
    MSDS
    Manual
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