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Leadgene Leadgene
Human GM-CSF ELISA Kit
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Catalog Number

LDG00028E

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  • Overview

    Description

    Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a Growth Factors due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.

    Test Principle

    Human GM-CSF ELISA kit is used to detect GM-CSF in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-human GM-CSF monoclonal antibody to the solid phase. Human GM-CSF in the sample conjugates on solid phase and then react with the HRP conjugated mouse anti-human GM-CSF monoclonal antibody. Subsequent wash steps will remove residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of nuclease level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Reactivity

      Human

      Application

      Sandwich ELISA analysis

    • Sensitivity

      0.0514 ng/mL

      Specificity

      Human GM-CSF

    • Assay Range

      0.078125 ng/mL-5 ng/mL

      Sample Type

      Human Serum / Human Plasma

    • Recovery

      Human Serum: 80-130%
      Human Plasma: 80-130%

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only.

    Typical data
    The following data are for demonstration only.

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

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  • Datasheet & Documents
    Datasheet
    MSDS
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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