Human Heregulin Beta 1, His Tag, E. coli

Catalog Number

LDG185PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 8.42 kDa.
    The protein migrates as 8 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is < 10 ng/mL.

    Endotoxin level

    <1.0 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Neuregulin-1 (NRG-1, also called heuregulin1 or neu differentiation factor) is a glycoprotein that belongs to the neuregulins family. Structurally, Neuregulin-1 harbors tissue-specific N terminal sequence, followed by immunoglobulin-like (Ig-like) domains, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. NRG1 has multiple isoforms produced by alternative splicing. Heregulin- β1 (HRG- β1) is one of the isoforms, has been reported to engage the development and survival of cardiomyocytes derived from embryonic stem (ES) cells via activating MAPK-ERK and PI3K-AKT pathways. Moreover, HRG- β1 plays a central role in promoting the proliferation of neuronal progenitors from embryonic neural stem cells.

    Synonyms

    NRG1 Beta 1, Heregulin-β1

  • Uniprot ID

    #Q02297-6

    Sequence Note

    Ser177-Glu241

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human Heregulin beta 1.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.