Human IL-2, His Tag, E. coli

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Catalog Number

LDG004PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of NaPi buffer, 0.018% SDS, pH 7.5.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 16.4 kDa.
    The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce proliferation in CTLL-2 cells. The ED₅₀ for this effect is <3 ng/mL. The specific activity of recombinant human IL-2 is >5 x 10⁶ IU/mg.

    Endotoxin level

    <0.05 EU per 1 µg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Interleukin-2 (IL-2) is a 15.5 kDa four bundle, immuno-modulatory cytokine which is expressed predominately by antigen-simulated CD4+ T cells, CD8+ T cells, natural killer (NK) cells, and dendritic cells (DC). IL-2 plays a fundamental role in promoting NK cell proliferation, cytotoxicity, and B cells differentiation. IL-2 modulate T cell differentiation programs, promoting naïve CD4+ T cell differentiation into T helper-1 (Th1) and T helper-2 (Th2) cells.

    Synonyms

    Interleukin-2, T-cell Growth Factors , TCGF

  • Uniprot ID

    #P60568

    Sequence Note

    Ala21-Thr153

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  The purity of recombinant human IL-2 is greater than 95% as determined by SEC-HPLC.

  SDS-PAGE analysis of recombinant human IL-2

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.