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Leadgene Leadgene
Human IL-28A, His Tag, E. coli
Catalog Number

LDG032PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 20.60 kDa.
      The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <20 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 28A (IL-28A) predicts a molecular mass of 22.3 kDa. IL-28 comes in two isoforms, IL-28A and IL-28B, and plays a role in immune defense against viruses, including the induction of an antiviral state by turning on Mx proteins, 2',5'-oligoadenylate synthetase as well as ISGF3G.

      Synonyms

      IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A

    • Uniprot ID

      #Q8IZJ0

      Sequence Note

      Pro27-Val200

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human IL-28A

    SDS-PAGE analysis of recombinant human IL-28A

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  • Datasheet & Documents
    MSDS
    Manual
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