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Leadgene Leadgene
Human Insulin Protein, E. coli
Catalog Number

LDG187PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      Tag free

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 5.81 kDa.
      The protein migrates as 5 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Insulin is a peptide hormone consisting of a double-chain structure (α, β) secreted by pancreatic β cells. It is the only known endogenous protein hormone capable of lowering blood sugar levels. Insulin not only regulates the intake, utilization, and storage of glucose, amino acids, and fatty acids in cells but also promotes the synthesis of glycogen, fats, and proteins.

      Synonyms

      INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

    • Uniprot ID

      P01308

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile 10 mM HCl to a concentration not more than 1-2 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human insulin protein

    SDS-PAGE analysis of recombinant human insulin protein

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  • Datasheet & Documents
    MSDS
    Manual
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