Human LAG-3, His Tag, HEK293

Species of Origin

Human

Expression system

HEK293

Affinity Tag

His Tag (C-term)

Buffer

Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

Purity

>95% as determined by SDS-PAGE analysis.

Molecular weight

The protein has a calculated MW of 47kDa.
The protein migrates as 60 kDa under reducing condition (SDS-PAGE analysis).

Endotoxin level

<0.1 EU per 1 μg of the protein by the LAL method.

Form

Lyophilized

Background

LAG-3, or Lymphocyte-activation gene 3, is a protein receptor expressed on the surface of certain immune cells, notably T cells and natural killer cells. It plays a pivotal role in regulating immune responses by inhibiting the activation and proliferation of T cells. LAG-3 is involved in immune tolerance and suppression, making it a promising target for immunotherapy in various diseases, including cancer and autoimmune disorders. Blocking LAG-3 can enhance anti-tumor immune responses, while agonists can suppress excessive immune activation. Studying LAG-3 function provides insights into immune regulation and therapeutic strategies.

Synonyms

CD233, FDC

Uniprot ID

P18627

Sequence Note

Met1-Leu450

Reconstitution

It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.2 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

Stability & Storage

This product is stable after storage at:

• -20°C for 12 months in lyophilized state from date of receipt.
• -20°C or -80°C for 1 month under sterile conditions after reconstitution.

Avoid repeated freeze/thaw cycles.