Human M-CSF, His Tag, E. coli

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Catalog Number

LDG093PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 13.34 kDa.
    The protein migrates as 13-26 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human M-CSF is approximately >2.5x 10⁸ IU/mg.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Macrophage Colony Stimulating Factor (M-CSF) is a 18.54 kDa member of hematopoietic Growth Factors with 159 amino acid residues. M-CSF produced by osteoblasts and osteoblast precursors. M-CSF stimulates the growth and differentiation of the monocyte lineage, and promotes the survival, proliferation, and functions of mature monocytes/macrophages.

    Synonyms

    Processed macrophage colony-stimulating factor 1, Lanimostim, Macrophage colony-stimulating factor 1

  • Uniprot ID

    #P09603

    Sequence Note

    Glu33-Ser190

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human M-CSF, His Tag, E. coli (LDG093PHE) induced NFS-60 cell proliferation, with the ED50 at 0.75 ng/mL.

  SDS-PAGE analysis of recombinant human M-CSF.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.