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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 13.28 kDa.
The protein migrates as 10 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
Macrophage migration inhibitory factor (MIF) is a pleiotropic inflammatory mediator, predicts a molecular mass of 12.5 kDa. MIF binds to CD74, a type II transmembrane protein, inducing its phosphorylation and the recruitment of CD44, which then activates SRC family non-receptor tyrosine kinases, leading to ERK1 /2 phosphorylation.
Synonyms
Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase
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Uniprot ID
#P14174
Sequence Note
Met1-Ala115
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![SDS-PAGE analysis of recombinant human MIF.]()
SDS-PAGE analysis of recombinant human MIF.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
