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Leadgene Leadgene
Human NF-L ELISA Kit
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Catalog Number

LDG00027E

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  • Overview

    Description

    Neurofilaments (NF) are assembled as heteropolymers, found exclusively in neurons, and serve as axonal scaffolding. They are composed of three subunits, including NF-L (68 kDa), NF-M (95 kDa), and NF-H (115 kDa), that are essential for axonal growth and maintenance. Elevated NF-L levels in serum and CSF (cerebrospinal fluid) have been correlated with axonal damage in Multiple Sclerosis patients, serving as a prognostic marker. In addition, plasma NF-L concentrations were highly significantly higher in patients with Alzheimer’s disease.

    Test Principle

    Human NF-L ELISA kit is used to detect NF-L in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-human NF-L monoclonal antibody to the solid phase. Human NF-L in the sample conjugates on solid phase and then react with the HRP conjugated mouse anti-human NF-L monoclonal antibody. Subsequent wash steps will remove residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of nuclease level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Reactivity

      Human

      Application

      Sandwich ELISA analysis

    • Sensitivity

      0.0629 ng/mL

      Specificity

      Human NF-L

    • Assay Range

      0.0313 ng/mL-2 ng/mL

      Sample Type

      Human Serum/ Human Plasma

    • Recovery

      Human Serum: 92-100%
      Human Plasma: 8X dilution: 83%

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only.

    Typical data
    The following data are for demonstration only.

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

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  • Datasheet & Documents
    Datasheet
    MSDS
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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