Human Noggin, Fc Tag, HEK293

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Catalog Number

LDG017PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    HEK293

  • Affinity Tag

    Human IgG1 Fc Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 49.11 kDa.
    The protein migrates as 58 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <0.05 µg/mL in the presence of 50 ng/mL of recombinant human BMP-4.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Noggin binds members of the transforming Growth Factors-beta (TGF beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP-4), which inactivates their activities. As a extracellular antagonist of BMP proteins, Noggin involves in the development of many body tissues, including nerve tissue, muscles, and bones. In addition, Noggin is able to inhibit chondrocyte differentiation through its interaction with GDF5.

    Uniprot ID

    Q13253

  • Sequence Note

    Gln28-Cys232

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human Noggin, Fc Tag, HEK293 (LDG017PHM), inhibits BMP-4-induced alkaline phosphatase production by ATDC5 cells, with an ED50 of <0.05 µg/mL.

  SDS-PAGE analysis of recombinant human Noggin

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.