Description
PD-L1 regulates immune responses by binding to the immunoinhibitory receptor PD-1 on activated T-cells and B-cells. This interaction can suppress T-cell responses by inducing apoptosis and arresting cell-cycle progression. In cancer, PD-L1's role in reducing antigen-specific T cells can promote tumor growth and immune evasion. As a result, PD-L1 is considered as a potential therapeutic target for autoimmune diseases and cancer.
Species of Origin
Human
Expression system
HEK293
Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 26 kDa.
The protein migrates as 30-35 kDa uder reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce adhesion in T-cell enriched PBMC cultures using a concentration range of 1-2 μg/mL.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
Background
Programmed death-ligand 1 (PD-L1) is an immune checkpoint protein expressed on various cell types, including tumor cells. It interacts with PD-1 on T cells to suppress immune responses, enabling tumor immune evasion. Recombinant PD-L1 with a His tag, expressed in HEK293 cells, is crucial for studying PD-1/PD-L1 interactions, developing immune checkpoint inhibitors, and conducting functional assays in cancer immunotherapy research. The use of HEK293 cells ensures proper folding and post-translational modifications, maintaining biological activity.
Synonyms
CD274, B7-H1
Uniprot ID
Q9NZQ7.1
Sequence Note
Phe19-Arg238
Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
Avoid repeated freeze/thaw cycles.
Measure by its ability to induce adhesion in T-cell enriched PBMC cultures using a concentration range of 1-2 μg/mL.
The ED₅₀ for this effect is 1.3 μg/mL.
SDS-PAGE analysis of recombinant human PD-L1.
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Disclaimer：For Research Use or Further Manufacturing Only.
