Human R-Spondin 1, His-SUMO Tag, HEK293

Catalog Number

LDG013PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    HEK293

  • Affinity Tag

    His-SUMO Tag (N-term)

    Concentration

    Please refer to the product label.

  • Buffer

    Liquid. Phosphate buffered saline, pH 7.4.

    Purity

    >95% as determined by SDS-PAGE analysis.

  • Molecular weight

    The protein has a calculated MW of 46.8 kDa.
    The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis).

    Activity

    R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL.

  • Endotoxin level

    <1 EU per 1 μg of the protein by the LAL method.

    Form

    Liquid

• Background

  • Background

    R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/ 6), subsequent clearing negative regulators ZNRF3 /RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.

    Synonyms

    Roof plate-specific spondin-1, hRspo1

  • Uniprot ID

    # Q2MKA7

    Sequence Note

    Ser21-Ala263

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long-term storage.

    Stability & Storage

    This product is stable after storage at:

    • -80°C for long-term storage under sterile conditions.

    Avoid repeated free-thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human R-Spondin 1.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.