Human RANKL, His Tag, E. coli

Catalog Number

LDG122PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 20.67 kDa.
    The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED₅₀ for this effect is <10 ng/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Receptor activator of NF-κB (RANK) ligand (RANKL) is type II transmembrane protein with an extracellular domain at the carboxy-terminus of TNF cytokine superfamily. RANKL is a 19.8 kDa protein containing 317 residues and high express in T cells and T cell rich organs, such as thymus and lymph nodes. RANKL-RANK (RANKL receptor) plays an important role in bone metabolism, dysregulation, and immune system

    Synonyms

    Receptor activator of nuclear factor kappa-B ligand, RANKL, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, TNF-related activation-induced cytokine, TRANCE, Tumor necrosis factor ligand superfamily member 11, TNFL11

  • Uniprot ID

    #O14788

    Sequence Note

    Glu143-Asp317

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human RANKL.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.