Human Sonic Hedgehog (C24II), Tag Free, HEK293

Catalog Number

LDG017PVM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    HEK293

  • Affinity Tag

    Tag Free

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 19.67 kDa.
    The protein migrates as 15-25 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.5-2 µg/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Sonic hedgehog protein (SHH) plays a critical role in development of embryonic morphogenesis which mediate the process of organogenesis and central nervous system. The sonic hedgehog signal pathway also plays an important role in cancerous tumors like embryonic cerebellar tumor, prostate cancer tumours and medulloblastoma. Shh binds the receptor (Ptc1) that induce the smo protein to activate the downstream pathway, which induce the shh target gene expression.

    Synonyms

    Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC

  • Uniprot ID

    #Q15465

    Sequence Note

    Gly25-Gly197

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of Human Sonic Hedgehog (C24II).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.