Human TGF beta 1, His Tag, HEK293

Catalog Number

LDG020PHM

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    HEK293

  • Affinity Tag

    His Tag (N-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.0.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 44.3 kDa.
    The protein migrates as approximately 12、40、50 kDa under reducing conditions (SDS-PAGE analysis) corresponding to mature TGFβ1, LAP protein, and inactive latent TGFβ1 respectively due to glycosylation

  • Activity

    Measure by its ability to inhibit the IL-4 dependent proliferation in TF-1 cells. The ED₅₀ for this effect is < 0.1 ng/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    The transforming Growth Factors beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions.

    Synonyms

    Transforming Growth Factors beta-1 proprotein

  • Uniprot ID

    P01137

    Sequence Note

    Met1-Ser390

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in 10 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human TGF beta 1 protein.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.