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Leadgene Leadgene
Human TGF beta 1, His Tag, HEK293
Catalog Number

LDG020PHM

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  • Specifications
    • Species of Origin

      Human

      Expression system

      HEK293

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 44.3 kDa.
      The protein migrates as approximately 12、40、50 kDa under reducing conditions (SDS-PAGE analysis) corresponding to mature TGFβ1, LAP protein, and inactive latent TGFβ1 respectively due to glycosylation

    • Activity

      Measure by its ability to inhibit the IL-4 dependent proliferation in TF-1 cells. The ED₅₀ for this effect is < 0.1 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      The transforming Growth Factors beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions.

      Synonyms

      Transforming Growth Factors beta-1 proprotein

    • Uniprot ID

      P01137

      Sequence Note

      Met1-Ser390

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in 10 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human TGF beta 1 protein.

    SDS-PAGE analysis of recombinant human TGF beta 1 protein.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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