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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
His Tag (C-term)
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 13.66 kDa.
The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human TGF beta 2 is > 5 x 10⁶ IU/mg.
Endotoxin level
<0.01 EU per 1 µg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
Transforming Growth Factors beta 2 (TGFβ-2) is a 12.85 kDa member of the epidermal Growth Factors with 113 amino acid residues. TGFβ-2 is expressed from throughout the body. TGFβ-2 is a regulator of cell proliferation, differentiation, apoptosis, cell plasticity and migration, etc. TGF-β-2 also associates with various kinds of diseases, such as cancer and tissue fibrosis.
Synonyms
Latency-associated peptide, LAP, Transforming Growth Factors beta-2, TGF-beta-2, Glioblastoma-derived T-cell suppressor factor, Transforming Growth Factors beta-2 proprotein
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Uniprot ID
P61812
Sequence Note
Ala303-Ser414
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 2 weeks under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![Human TGF Beta 2, His Tag, E. coli (LDG091PHE) inhibited IL-4-induced HT-2 cell proliferation, with the ED50 at 0.17 ng/mL.]()
Human TGF Beta 2, His Tag, E. coli (LDG091PHE) inhibited IL-4-induced HT-2 cell proliferation, with the ED50 at 0.17 ng/mL.
![SDS-PAGE analysis of recombinant Human TGF Beta 2.]()
SDS-PAGE analysis of recombinant Human TGF Beta 2.
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Review
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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