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Leadgene Leadgene
Human TNF Beta, His Tag, E. coli
Catalog Number

LDG115PHE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 18.65 kDa.
      The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 10⁸ IU/mg.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Human TNF beta (Lymphotoxin-alpha) is a 22 kDa cytokine with 205 amino acid residues. TNF beta belongs to tumor necrosis factor (TNF) superfamily as TNF alpha. Binding to lymphotoxin-β receptor (LtβR), canonical and non-canonical NF-κB signal pathway will be activated. TNF beta is related to apoptosis and inflammatory signals

      Synonyms

      Tumor Necrosis Factor Beta, TNF Beta, Lymphotoxin-alpha, LT-alpha, Tumor necrosis factor ligand superfamily member 1, TNFSF1

    • Uniprot ID

      #P01374

      Sequence Note

      Leu1-Leu171

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human TNF Beta.

    SDS-PAGE analysis of recombinant human TNF Beta.

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  • Datasheet & Documents
    MSDS
    Manual
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