Human VEGF165, His Tag, E. coli

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Catalog Number

LDG168PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 20.11 kDa.
    The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce HUVEC cells proliferation. The ED₅₀ for this effect is <12 ng/mL. The specific activity of recombinant human VEGF165 is approximately >5 x 10⁵ IU/mg.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Vascular Endothelial Growth Factors 165(VEGF165) is a potent growth and angiogenic cytokine which belongs to the VEGF family, includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Human VEGF165 is an abundant glycosylated cytokine composed of two identical 165 amino acid chains. Human VEGF165 plays an important role in embryonic vasculogenesis, angiogenesis and neurogenesis.

    Synonyms

    Vascular permeability factor, VPF, Vascular endothelial Growth Factors A

  • Uniprot ID

    # NP_001165097

    Sequence Note

    Ala27-Arg191

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human VEGF165, His Tag, E. coli (LDG168PHE) induced HUVEC cell proliferation, with the ED50 at 4.51 ng/mL.

  SDS-PAGE analysis of recombinant human VEGF165.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.