Description
Interleukin-8 (IL-8) is a multifunctional chemokine produced by macrophages and several cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. IL-8 is an important protein related to inflammation, that attracts neutrophils and other immune cells to the site of infection. IL-8 also participates in neutrophil chemotactic effects, mitogenesis, leukocyte activation, and calcium homeostasis.
Leadgene® IL-8 ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of IL-8 level in human serum sample. The IL-8 ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.
Test Principle
IL-8 ELISA kit is used to detect IL-8 in human serum and recombinant human IL-8 proteins by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-IL-8 monoclonal antibody to the solid phase. IL-8 in the serum samples conjugates on solid phase and then react with the HRP conjugated mouse anti-IL-8 monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of IL-8 level is accomplished by comparing the absorbance with standards curve.
