Inorganic Pyrophosphatase (Yeast)

Catalog Number

LDG0007RI

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• Overview

  Description

  Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P₂O₇⁻⁴ + H₂O + PPase → 2HPO₄⁻²). PPase requires divalent metal cation (Mg²⁺) for its enzymatic activity.

  Product Note

  Inorganic Pyrophosphatase (Yeast) requires divalent metal cation (Mg² ⁺) for its enzymatic activity. This enzyme is widely used in RNA IVT reaction.

• Specifications

  • Expression system

    Escherichia coli

    Application

    In vitro transcription, RNA amplification, miRNA and siRNA synthesis

  • Concentration

    0.1 U/µL

    Buffer

    Inorganic pyrophosphatase is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and 50% glycerol.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Unit Definition

    One unit is defined as the amount of the enzyme hydrolysis 1 μmol of inorganic pyrophosphate in 1 minutes at 25°C.

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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