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Expression system
Escherichia coli
Activity
≥200 U/mg
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Unit Definition
One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute under the conditions described below.
Form
Lyophilized (Yellowish amorphous powder)
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Specifications
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Background
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Synonyms
LOX, LctO, Lactic oxygenase, Lactic oxidase, Lactate monooxygenase, L-lactate oxidase
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Instruction
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Reconstitution
It is recommended to weight and reconstitute 10 mg of lyophilized powder in 200 μL double-distilled water directly (final activity is 10 U/ μL) and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable at -20°C for long-term storage under sterile conditions.
Avoid repeated free-thaw cycles.
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Image
![pH stability of LOX. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition at 25°C for 16 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.5-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.]()
pH stability of LOX.
The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition at 25°C for 16 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.5-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.![Thermal stability of LOX. The enzyme powder was reconstituted by double-distilled water and treated with different temperatures for 10 minutes. Final concentration: 10 U/ mL.]()
Thermal stability of LOX.
The enzyme powder was reconstituted by double-distilled water and treated with different temperatures for 10 minutes. Final concentration: 10 U/ mL.![pH activity of LOX. The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.5-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.]()
pH activity of LOX.
The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.5-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.![Temperature activity of LOX. The enzyme reactions in 20 M K-Phosphate buffer, pH 7.5, were carried out under different temperatures.]()
Temperature activity of LOX.
The enzyme reactions in 20 M K-Phosphate buffer, pH 7.5, were carried out under different temperatures.1/4 -
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Publication
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Datasheet & Documents
1/2
Disclaimer：For Research Use or Further Manufacturing Only.
