LeadGMP® BspQI

Expression system

Escherichia coli

Concentration

10 U/μL

Buffer

20 mM Tris-HCl, 500 mM KCl, 0.1 mM EDTA, 1 mM DTT, 500 μg/ml rAlbumin, 50% Glycerol, 0.1% Triton X-100, pH 7

Activity

One unit of BspQI is defined as the amount of enzyme that cleave 1 μg λDNA in a total reaction volume of 50 μL at 50°C for 1h.

Endotoxin level

<0.1 EU per 1 mL of the enzyme by the LAL method.

Mycoplasma

Not detected

Form

Liquid

Background

BspQI is a restriction enzyme derived from bacteria that recognizes and cuts specific DNA sequences. Its recognition sequence is 5'-GCTCTTC-3', with the cleavage site located downstream from the recognition sequence. BspQI is commonly used in molecular biology applications such as genome editing, cloning, and DNA mapping due to its ability to precisely cut DNA at defined sites, facilitating further analysis and manipulation of the DNA molecules.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

Stability & Storage

This product is stable after storage at:

• -20°C for long-term storage under sterile conditions.

Manufacturing specifications

LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

• Animal-free reagent and laboratory
• Manufactured and tested under GMP guideline
• Testing and traceability of raw material
• Records of the maintenance and equipment calibration
• Personnel training records
• Batch-to-batch consistency
• Documentation of QA control and process changes
• Manufactured and tested under an ISO 13485:2016 certified quality management system
• Stability monitor of product shelf-life