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Leadgene Leadgene
LeadGMP® Endoglycosidase H (Endo H)
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Catalog Number

LDG002R-GMP

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  • Overview

    Product Note

    • After thawing, the buffers may crystallize, which is a normal occurrence. Warm crystallized buffer until the salt crystals return to solution. Ensure that your components return to RT before use in the assay.
    • Please fine-tune the input sample volume to find the optimal condition for your assay.
    • Once optimize for the cleavage condition, the cleavage reactions can be scaled up to cleave a large amount of the target fusion protein.

    Components

    Items

    Quantity

    LeadGMP® Endoglycosidase H (Endo H) (500 U/μL)

    1 vial (50,000 U)

    10× Glycoprotein Denaturing Buffer

    1 vial (1 mL)

    10× Reaction buffer

    1 vial (1 mL)

    • LeadGMP® Endoglycosidase H (Endo H) (500 U/μL)

      Quantity

      1 vial (50,000 U)

    • 10× Glycoprotein Denaturing Buffer

      Quantity

      1 vial (1 mL)

    • 10× Reaction buffer

      Quantity

      1 vial (1 mL)

  • Specifications
    • Species of Origin

      Streptomyces plicatus

      Expression system

      Escherichia coli

    • Concentration

      500 U/μL

      Buffer

      20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Activity

      One unit of Endoglycosidase H cleaves > 95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 μL at 37°C for 1 h.

    • Endotoxin level

      <0.05 EU per 1 µg of the protein by the LAL method.

      Mycoplasma

      Not detected

    • Form

      Liquid

  • Background
    • Background

      Protein glycosylation is a complex posttranslational modification that manipulates biological activity such as protein folding, intracellular trafficking, stability, and half-life, affecting protein function. Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C for long-term storage under sterile conditions. Avoid repeated free-thaw cycles.
    • Manufacturing specifications

      LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life
  • Image
    Endo H cleaves high mannose and hybrid-type glycans.

    Endo H cleaves high mannose and hybrid-type glycans.

    The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

    The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

    SDS-PAGE analysis of recombinant Endoglycosidase H

    SDS-PAGE analysis of recombinant Endoglycosidase H

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  • Datasheet & Documents

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