LeadGMP® Endoglycosidase H (Endo H)

Species of Origin

Streptomyces plicatus

Expression system

Escherichia coli

Concentration

500 U/μL

Buffer

20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5

Purity

>95% as determined by SDS-PAGE analysis.

Activity

One unit of Endoglycosidase H cleaves > 95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 μL at 37°C for 1 h.

Endotoxin level

<0.05 EU per 1 µg of the protein by the LAL method.

Mycoplasma

Not detected

Form

Liquid

Background

Protein glycosylation is a complex posttranslational modification that manipulates biological activity such as protein folding, intracellular trafficking, stability, and half-life, affecting protein function. Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

Stability & Storage

This product is stable after storage at:

• -20°C for long-term storage under sterile conditions. Avoid repeated free-thaw cycles.

Manufacturing specifications

LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

• Animal-free reagent and laboratory
• Manufactured and tested under GMP guideline
• Testing and traceability of raw material
• Records of the maintenance and equipment calibration
• Personnel training records
• Batch-to-batch consistency
• Documentation of QA control and process changes
• Manufactured and tested under an ISO 13485:2016 certified quality management system
• Stability monitor of product shelf-life