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Species of Origin
Human
Expression system
Escherichia coli
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Affinity Tag
Tag Free
Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>97% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 18.2 kDa.
The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED₅₀ for this effect is <6 ng/mL. The specific activity of recombinant LeadGMP® human IL-18 is > 1 x 10⁷ IU/mg, which is calibrated against the human IL-18 WHO Reference Reagent (NIBSC code: 03/200).
Endotoxin level
<0.05 EU per 1 µg of the protein by the LAL method.
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Mycoplasma
Not detected
Form
Lyophilized
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Specifications
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Background
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Background
Interleukin-18 (IL-18) belongs to the IL-1 superfamily and is constitutively produced by macrophages, dendritic cells (DCs) and other cells. IL-18 binds to the receptor of IL-18 (IL-18R) and initiate the recruitment and heterodimerization of the IL-18RAcP, leading to downstream activation of NF-κB. After stimulation with IL-18, multiple cytokines including IFN-γ, IL-13, IL-4, IL-8, and GM-CSF and both Th1 and Th2 lymphokines could be produced by different cells. As an immunoregulaory cytokine, IL-18 can promote development of T helper 1 (Th1) cells, which maximizes the production of IFN by synergistically stimulating to mature Th1 effectors in combination with IL-12. On the contrary, it inhibits the production of the anti-inflammatory cytokine IL-10. In addition, IL-18 exhibits multiple proinflammatory functions, such as increases in cell adhesion molecules, nitric oxide synthesis, and chemokine production.
Synonyms
Interleukin-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma , IL-1 gamma
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Uniprot ID
Q14116
Sequence Note
Tyr37-Asp193
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Manufacturing specifications
LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
- Animal-free reagent and laboratory
- Manufactured and tested under GMP guideline
- Testing and traceability of raw material
- Records of the maintenance and equipment calibration
- Personnel training records
- Batch-to-batch consistency
- Documentation of QA control and process changes
- Manufactured and tested under an ISO 13485:2016 certified quality management system
- Stability monitor of product shelf-life
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Image
![LeadGMP® Human IL-18 Protein, Tag Free, E. coli (LDG025PHE-GMP) induces IFN gamma secretion in KG-1 cells, with the ED50 < 6 ng/mL.]()
LeadGMP® Human IL-18 Protein, Tag Free, E. coli (LDG025PHE-GMP) induces IFN gamma secretion in KG-1 cells, with the ED50 < 6 ng/mL.
![SDS-PAGE analysis of LeadGMP® IL-18, Human]()
SDS-PAGE analysis of LeadGMP® IL-18, Human
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
