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Leadgene Leadgene
Mouse Activin B, His Tag, E. coli
Catalog Number

LDG083PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 13.71 kDa.
      The protein migrates about 12 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is <1 ng/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Activin B is the cytokine of the TGF beta family, which is a 12.9 kDa protein containing 116 amino acid residues. Activin B has the wide variety of biological function including stem cell differentiation, inflammation, bone remodeling and neural development. It also has a role in regulation of FSH secretion. Activin B induces the phosphorylation of Smad which binds the smad binding element.

      Synonyms

      Inhibin beta B chain, Activin beta-B chain

    • Uniprot ID

      #Q04999

      Sequence Note

      Gly297-Ala411

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse Activin B.

    SDS-PAGE analysis of recombinant mouse Activin B.

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  • Datasheet & Documents
    MSDS
    Manual
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