Mouse ENO1, His Tag, E. coli

Catalog Number

LDG091PME

Datasheet

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• Specifications

  • Species of Origin

    Mouse

    Expression system

    Escherichia coli

  • Affinity Tag

    His Tag (N-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS with 7 mM MgSO₄, pH 7.2

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 47.82 kDa.
    The protein migrates about 48 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    Enolase 1 (ENO1) also named alpha-enolase which is a glycolytic enzyme that plays the key reactions of glycolysis. It could convert the NAD+ to NADH by producing two ATP. ENO1 is a homodimer which is composed by two isozymes of enolase (2 α, 2 ν, or 2 β). It also plays an important role in cancer model which can promote tumor cell proliferation and migration by the PI3K signaling pathway. ENO1 is a biomarker of prognostic and diagnostic cancer.

    Synonyms

    Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE

  • Uniprot ID

    #P17182

    Sequence Note

    Ser2-Lys434

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant mouse ENO1.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.