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Leadgene Leadgene
Mouse Nectin-4, His Tag, CHO
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Catalog Number

LDG089PMM

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  • Specifications
    • Species of Origin

      Mouse

      Expression system

      CHO

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 35.12 kDa.
      The protein migrates as 38-40 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is <1.5 μg/mL.

      Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Mycoplasma

      Not detected

      Form

      Lyophilized

  • Background
    • Background

      Nectin-4 is a murine cell adhesion molecule, involved in immune signaling and tumorigenesis, similar to its human counterpart.

      Synonyms

      Nectin-4, Ig superfamily receptor LNIR, Nectin cell adhesion molecule 4, Poliovirus receptor-related protein 4, Cleaved into: Processed poliovirus receptor-related protein 4

    • Uniprot ID

      Q8R007

      Sequence Note

      Gly31-Ser347

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.2 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      "This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles."

  • Image
    Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is &lt;1.5 μg/mL. Activity analysis of recombinant Mouse Nectin-4, His Tag, CHO.

    Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is <1.5 μg/mL.
    Activity analysis of recombinant Mouse Nectin-4, His Tag, CHO.

    SDS-PAGE analysis of recombinant mouse Nectin4.

    SDS-PAGE analysis of recombinant mouse Nectin4.

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  • Datasheet & Documents
    MSDS
    Manual
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