Mouse TNFR-1, His Tag, CHO

More Images

Catalog Number

LDG007PMM

Datasheet

More Documents

Loading package & price...

COA下載

• Specifications

  • Species of Origin

    Mouse

    Expression system

    CHO

  • Affinity Tag

    His Tag (C-term)

    Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 21.15 kDa.
    The protein migrates as 30-40 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measured by its ability to inhibit TNF-alpha mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of actinomycin D and 0.25 ng/mL recombinant mouse TNF-alpha, with the ED50 being < 0.8 µg/mL.

    Endotoxin level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    TNFR-1, or Tumor Necrosis Factor Receptor 1, is a transmembrane protein receptor found on the surface of various cell types. It serves as a key mediator in the signaling pathway activated by Tumor Necrosis Factor-alpha (TNF-alpha), a cytokine involved in inflammation and immune response regulation. Upon binding with TNF-alpha, TNFR-1 triggers downstream signaling cascades that regulate cell survival, apoptosis, and inflammation. Dysregulation of TNFR-1 signaling has been implicated in autoimmune diseases, inflammatory conditions, and cancer. Understanding the molecular mechanisms of TNFR-1 activation is crucial for developing targeted therapies for diseases involving TNF-alpha dysregulation.

    Synonyms

    Tumor necrosis factor receptor 1 (TNF-R1), Tumor necrosis factor receptor type I (TNF-RI; TNFR-I), p55, p60, CD120a

  • Uniprot ID

    NP_035739.2

    Sequence Note

    Met1-Ala212

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.2 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Mouse TNFR-1 Protein, His Tag, CHO (LDG007PMM) inhibits TNF-alpha mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of actinomycin D and 0.25 ng/mL recombinant mouse TNF-alpha, with the ED50 being < 0.8 µg/mL.

  SDS-PAGE analysis of recombinant mouse TNFR-1.

  1

  /

  2

  Total   (2)

• Review

  

  Help others learn more about this product. Use the link below to share your experience.

  Submit a Review

• Publication

  

  There are currently no publications. Use the link below to let us know.

  Submit a Publication

• Datasheet & Documents

  Datasheet

  MSDS

  Manual

  1

  /

  1

Disclaimer：For Research Use or Further Manufacturing Only.