Description
Nuclease is a genetically engineered endonuclease from Serratia marcescens, it is produced and purified from E. coli. The enzyme is a non-specific nuclease, which effectively degrades single-stranded, double-stranded, linear, and circular DNA and RNA over a wide temperature and pH range.
Expression system
Escherichia coli
Concentration
250 U/μL
Buffer
Nuclease is supplied in 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 2 mM MgCl₂ and 50% glycerol
Purity
>95% as determined by SDS-PAGE analysis.
Unit Definition
One unit of Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA.
Form
Liquid
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
Avoid repeated free-thaw cycles.
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Disclaimer：For Research Use or Further Manufacturing Only.
