Description
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the genus Betacoronavirus, family Coronaviridae that causes coronavirus disease 2019 (COVID-19). SARS-CoV-2 is an enveloped positive-sense single-stranded RNA virus. Like other coronaviruses, SARS-CoV-2 has four structural proteins: spike, envelope, membrane and nucleocapsid. The nucleocapsid protein (NP) is the most abundant protein in coronavirus that encapsulates the genomic RNA and often used as a marker in diagnostic assays due to its high immunogenicity.
Leadgene® SARS CoV-2 Nucleocapsid Protein ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of SARS-CoV-2 NP level in cell culture medium, nasal swab and saliva. The SARS-CoV-2 Nucleocapsid Protein ELISA kit is for research use only (RUO). Not suitable for use in therapeutic procedures.
Test Principle
Leadgene® SARS CoV-2 Nucleocapsid Protein ELISA kit is used to detect SARS CoV-2 NP in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-SARS CoV-2 NP monoclonal antibody to the solid phase. SARS CoV-2 NP in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-SARS CoV-2 NP monoclonal antibody. Subsequent wash steps will remove unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of SARS CoV-2 NP level is accomplished by comparing the absorbance value with standard curve.
