SARS-CoV Nucleocapsid Protein, His-SUMO Tag, HEK293

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Catalog Number

LDG005PVM

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Specifications
- Species of Origin
  
  SARS-CoV
  
  Expression system
  
  HEK293
- Affinity Tag
  
  His-SUMO Tag (N-term)
  
  Buffer
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- Purity
  
  >98% as determined by SDS-PAGE analysis.
  
  Molecular weight
  
  The protein has a calculated MW of 57.57 kDa.
  The protein migrates about 75 kDa under reducing condition (SDS-PAGE analysis).
- Form
  
  Lyophilized
Background
- Background
  
  SARS‑CoV‑2 is a kind of coronavirus which full name is severe acute respiratory syndrome coronavirus 2. SARS‑CoV‑2 is contagious that causes the respiratory diseases and lung diseases which make difficulty breathing. SARS‑CoV‑2 do the spillover event in 2019 because it has genetic diversity. SARS-CoV-2 is composed by four subunits (spike, envelope, membrane and nucleocapsid proteins). Its RNA genome is encapsulated with nucleocapsid protein. The viral envelope is comprised of spike, envelope and membrane protein. SARS‑CoV‑2 has high affinity to ACE2, which is highly expression in intestines, kidney, and heart.
  
  Synonyms
  
  Nucleoprotein, N, Nucleocapsid protein, NC Protein N
- Uniprot ID
  
  #P59595
  
  Sequence Note
  
  Met1-Ala422
Instruction
- Reconstitution
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- Stability & Storage
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
Image

ELISA titration of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG) (Leadgene cat. 17901).

WB analysis of recombinant SARS-CoV nucleocapsid protein.
Lane 1: Human anti-SARS-CoV & CoV-2 NP Antibody (IgG), 0.5 μg/mL (Leadgene cat. 17901)
Lane 2: Human anti-SARS-CoV & CoV-2 NP Antibody (IgM), 0.5 μg/mL (Leadgene cat. 18301)

SDS-PAGE analysis of recombinant SARS-CoV nucleocapsid protein.

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Datasheet & Documents

Datasheet

MSDS

Manual

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